Cell heterogeneity, rather than the cell storage solution, affects the behavior of mesenchymal stem cells in vitro and in vivo

Abstract Background Mesenchymal stem cells (MSCs) have to be expanded in vitro reach a sufficient cell dose for the treatment of various diseases. During process expansion, some obstacles remain overcome. The purpose this study was investigate effects storage solutions and heterogeneity on behavior MSCs vivo. Methods Umbilical cord (UC-MSCs) similar sizes within normal ranges were suspended three different solutions, phosphate buffer solution, saline, Dulbecco’s modified Eagle medium. Then, ultrastructure, viability, safety these compared. Other two UC-MSC populations categorized based their mean diameters. proliferation, immunosuppression, hepatic differentiation potential, number senescent investigated survival rates mice after infusion UC-MSCs Results For apoptosis rates, similar, no differences observed. Cells with diameter 19.14 ± 4.89 μm as larger population, 15.58 3.81 smaller population. population significantly than that ( p < 0.01). Smaller had more powerful proliferation immunosuppressive potential higher nucleus-cytoplasm ratio those large UC-MSCs. positive β-galactosidase staining receiving 1 × 10 6 or 2 100%, both which same amounts cause mouse death explored it found accumulated pulmonary capillary dead mice. Conclusion Different showed significant behavior, whereas quite prevalent MSC might limit application. Hence, is necessary establish precise standardization culture-expanded MSCs.